UL-771

Material Data Sheet

His6-Pro-SUMO4

Price: $150.00
Catalog #: UL-771

His6-Pro-SUMO4

Price: 
150
Quantity: 
500 µg
Data Sheet: 
application/pdf iconUL-771_hisproSUMO4.pdf

All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein.  His6-Pro-SUMO-4 (109 amino acids) is the inactive precursor of His6-SUMO-4 (107 amino acids) and is processed at the C-terminus.  The resulting SUMO-4 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions.  This protein can be used as a negative control in sumolyation reactions.  This His6 tag is at the N-terminus.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT.  Actual concentration varies with lot number.
MW: 
12.4 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations are 10-50 µM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity: 
> 95% by SDS-PAGE

Literature

References: 

Bohren, K., et al. (2004) J. Biol. Chem. 279: 27233-27238.

Guo, D., et al. (2005) Nature Genet. 36: 837-841.

Park, Y., et al (2005) Nature Genet. 37: 112.

Qu, H., et al (2005) Nature Genet. 37: 111-112.

Smyth, D.J., et al (2005) Nature Genet. 37: 328.

Wang, C.Y., Yang, P., She, J.X. (2005) Nature Genet. 37: 112-113.