UL-768

Material Data Sheet

SUMO3 AMC

Price: $269.00
Catalog #: UL-768

SUMO3 AMC

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

Fluorogenic substrate for SUMO-3 hydrolases based on the carboxy-terminus derivatization of SUMO-3 with 7-amido-4-methylcoumarin (AMC). SUMO-2 AMC is useful for studying SUMO-3 hydrolases (SENPs) when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein contains an N-terminal HA tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM Hepes pH 6.5, 200 mM NaCl, 10 % glycerol.
MW: 
11 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM. Release of AMC fluorescence can be monitored using Ex380 nm and Em460 nm wavelengths respectively.

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kim I.L., et al. (2000) J.Biol.Chem. 275:14102-14106
Suzuki T., et al. (1999) J.Biol.Chem. 274:31131-31134
Li S-J. and Hochstrasser M. (2003) J.Cell.Biol. 160:1069-1081
Mikolajczyk J., et al. (2007) J Biol.Chem. 282:26217-26227
Mukhupadhyay D, and Dasso M. (2007) Tren.Biochem.Sci. 32:286-295
Wilkinson K.D., et al. (2005) Meth.Enz. 399 :37-51