UL-766

Material Data Sheet

SUMO3 Fluorescein

Price: $195.00
Catalog #: UL-766

SUMO3 Fluorescein

Price: 
$195
Quantity: 
50 µg

SUMO-3 modified with fluorescein via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. This labeled SUMO-3 allows for direct detection spectophotometrically with higher efficiency and sensitivity
than with antibodies.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
10.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein-SUMO-3 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Lapenta V., et al. (1997) Genomics 40:362-366
Gill G. (2004) Genes.Dev. 18:2046-2059
Meluh P.B. and Koshland D. (1995) Mol. Biol. Cell 6: 793-807
Saitoh H. and Hinchey J. (2000) J.Biol. Chem. 275:6252-6258
Su H.L. and Li S. S.-L. (2002) Gene 296: 65-73
Subramanian L., et al. (2003) J.Biol. Chem. 278:9134-9141
Yeh E.T.H., et al. (2000) Gene 248:1-14