UL-765

Material Data Sheet

SUMO3 agarose

Price: $150.00
Catalog #: UL-765

SUMO3 agarose

Price: 
$150
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconUL-765 SUMO3 agarose.pdf
Stock: 

0.5 ml of SUMO3 agarose is supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

SUMO3 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of SUMO3 interacting proteins such as the SUMO activating E1 enzyme, the SUMO carrier enzyme Ubch9, SUMO E3 ligases, SENPs and other proteins/enzymes that have an affinity for SUMO proteins.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Desterro J.M. et al. (1997) FEBs. Lett. 417:297-300
Dohmen R.J. (2004) Biochem. Biophys. Acta. 1695:113-131
Li T. et al. (2006) Arch.Biochem.Biophys. 453:68-72
Rodriguez M.S et al. (2001) J. Biol. Chem. 276:12654
Su H-L. et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J. Biol. Chem. 276:35368-35374