UL-764

Material Data Sheet

SUMO3 Biotin

Price: $95.00
Catalog #: UL-764

SUMO3 Biotin

Price: 
$95
Quantity: 
50 µg
Data Sheet: 
SUMO-3 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. Biotinylated SUMO-3 can be detected using avidin-linked reagents for higher efficiency and sensitivity than with antibodies.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
10.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations are 10-50 μM depending on conditions.

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem. Biophys. Res. Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat. Rev. 4:690-699
Su H-L. et al. (2002) Gene. 296:65-73
Tatham M.H. et al. (2001) J. Biol. Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene. 248:1-14