UL-764

Material Data Sheet

SUMO3 Biotin

Price: $80.00
Catalog #: UL-764

SUMO3 Biotin

Price: 
$80
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-764 biotSUMO3_.pdf
Stock: 

X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.

MW: 
10.5 kDa
Purity: 
> 95% by SDS-PAGE
SUMO-3 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. Biotinylated SUMO-3 can be detected using avidin-linked reagents for higher efficiency and sensitivity than with antibodies.

Use & Storage

Use: 
Typical in vitro concentrations are 10-50 μM depending on conditions.
Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem. Biophys. Res. Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat. Rev. 4:690-699
Su H-L. et al. (2002) Gene. 296:65-73
Tatham M.H. et al. (2001) J. Biol. Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene. 248:1-14