UL-763

Material Data Sheet

His6-SUMO3

Price: $159.00
Catalog #: UL-763

His6-SUMO3

Price: 
$159
Quantity: 
500 µg
Data Sheet: 

Also known as: His6-SUMO-3 (SMT3B)

The ubiquitin-like SUMO-3 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-3 is derived from the precursor pro-SUMO-3 (Accession # NM_006936). Human SUMO-3 shares 47% and 87% identity with SUMO-1 and SUMO-2 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
MW: 
11.4 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Lapenta V., et al. (1997) Genomics 40:362-366
Gill G. (2004) Genes.Dev. 18:2046-2059
Meluh P.B. and Koshland D. (1995) Mol.Biol.Cell 6: 793-807
Saitoh H. and Hinchey J. (2000) J.Biol.Chem. 275:6252-6258
Su H.L. and Li S.S.-L. (2002) Gene 296: 65-73
Subramanian L., et al. (2003) J.Biol. Chem. 278:9134-9141
Yeh E.T.H.,et al. (2000) Gene 248:1-14