UL-763

Material Data Sheet

His6-SUMO3

Price: $150.00
Catalog #: UL-763

His6-SUMO3

Price: 
$150
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL763_hisSUMO3.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.

MW: 
11.4 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6-SUMO-3 (SMT3B)

The ubiquitin-like SUMO-3 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-3 is derived from the precursor pro-SUMO-3 (Accession # NM_006936). Human SUMO-3 shares 47% and 87% identity with SUMO-1 and SUMO-2 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Use & Storage

Use: 
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 
Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Lapenta V., et al. (1997) Genomics 40:362-366
Gill G. (2004) Genes.Dev. 18:2046-2059
Meluh P.B. and Koshland D. (1995) Mol.Biol.Cell 6: 793-807
Saitoh H. and Hinchey J. (2000) J.Biol.Chem. 275:6252-6258
Su H.L. and Li S.S.-L. (2002) Gene 296: 65-73
Subramanian L., et al. (2003) J.Biol. Chem. 278:9134-9141
Yeh E.T.H.,et al. (2000) Gene 248:1-14