UL-761

Material Data Sheet

His6-Pro-SUMO3

Price: $150.00
Catalog #: UL-761

His6-Pro-SUMO3

Price: 
$150
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL761_hisproSUMO3.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.

MW: 
13.8 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6 Pro-SUMO-3 (SMT3B)

All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-3 (103 amino acids) is the inactive precursor of SUMO-3 (92 amino acids) and is processed at the C-terminus by SUMO-3 specific proteases (SENPs). The resulting SUMO-3 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumolyation reactions or as a substrate for SENPs. This His6 tag is at the N-terminus. NCBI Accession # NM_006936..

Use & Storage

Use: 
Typical in vitro concentrations are 10-50 μM depending on conditions. 
Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Lapenta V., et al. (1997) Genomics 40:362-366
Gill G. (2004) Genes.Dev. 18:2046-2059
Meluh P.B. and Koshland D. (1995) Mol.Biol.Cell 6: 793-807
Saitoh H. and Hinchey J. (2000) J.Biol.Chem. 275:6252-6258
Su H.L. and Li S.S.-L. (2002) Gene 296: 65-73
Subramanian L., et al. (2003) J.Biol. Chem. 278:9134-9141
Yeh E.T.H.,et al. (2000) Gene 248:1-14