Material Data Sheet
Pro-SUMO3Catalog #: UL-760
All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-3 (103amino acids) is the inactive precursor of SUMO-3 (92 amino acids) and is processed at the C-terminus by SUMO-3 specific proteases (SENPs). The resulting SUMO-3 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in SUMOylation reactions or as a substrate for SENPs. NCBI Accession # NM_006936.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
Use & Storage
Typical in vitro concentrations are 10-50 μM depending on conditions.
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
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