UL-759

Material Data Sheet

HA-SUMO2 Vinyl Sulfone

Price: $250.00
Catalog #: UL-759

HA-SUMO2 Vinyl Sulfone

Price: 
250
Quantity: 
50 µg
Data Sheet: 
application/pdf iconUL759 HA-SUMO2 VS.pdf

Also known as: HA-SUMO2-VS

This N-terminal HA-tagged SUMO is a potent, irreversible and specific inhibitor of SUMO-specific proteases (SENPs). This protein inhibits the hydrolysis of poly-SUMO chains on substrate proteins in vitro and thus enhances poly-SUMO chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of SENP activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

Product Information

Stock: 
X mg/ml (XμM) in 50 mM Hepes pH 7.0, 150 mM NaCl, 10 % glycerol. Concentration varies with Lot #.
MW: 
10.6 kDa
Purity: 
> 90%

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 μM.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159

Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291

Di Bacco A., et al. (2006) Mol.Cell.Bio. 26: 4489-4498

Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95

Kessler B.M. (2006) Exp.Rev.Proteomics. 3 :213-221

Love K.R, et al. (2007) Nat.Chem.Biol. 3:697-705

Wang G., et al. (2003) Org.Lett. 5: 737-740