UL-758

Material Data Sheet

SUMO2 AMC

Price: $250.00
Catalog #: UL-758

SUMO2 AMC

Price: 
250
Quantity: 
50 µg
Data Sheet: 
application/pdf iconUL-758 SUMO2 amc.pdf

Fluorogenic substrate for SUMO-2 hydrolases based on the carboxy-terminus derivatization of SUMO-2 with 7-amido-4-methylcoumarin (AMC). SUMO-2 AMC is useful for studying SUMO-2 hydrolases (SENPs) when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein contains an N-terminal HA tag.

Product Information

Stock: 
X mg/ml (XμM) in 50 mM Hepes pH 6.5, 200 mM NaCl, 10 % glycerol. Actual concentration will vary with specific Lot #.
MW: 
11 kDa
Purity: 
> 95% by HPLC

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kim I.L., et al. (2000) J.Biol.Chem. 275:14102-14106
Suzuki T., et al. (1999) J.Biol.Chem. 274:31131-31134
Li S-J. and Hochstrasser M. (2003) J.Cell.Biol. 160:1069-1081
Mikolajczyk J., et al. (2007) J Biol.Chem. 282:26217-26227
Mukhupadhyay D, and Dasso M. (2007) Tren.Biochem.Sci. 32:286-295
Wilkinson K.D., et al. (2005) Meth.Enz. 399 :37-51