UL-756

Material Data Sheet

SUMO2 Fluorescein

Price: $195.00
Catalog #: UL-756

SUMO2 Fluorescein

Price: 
$195
Quantity: 
50 µg

SUMO-2 modified with fluorescein via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. This labeled SUMO-2 allows for direct detection spectophotometrically with higher efficiency and sensitivity
than with antibodies.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
10.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein-SUMO-2 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380, 1993.
Dai K.-S. and Liew C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Kamitani T., et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V. et al. (1997) Genomics 40: 362-366
Huang W-C. et al. (2004) Eur. J. Biochem. 271: 4114-4122
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Su H.L. and Li. S.S.-L. (2002) Gene. 296: 65-73
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Yeh E.T.H., et al. (2000) Gene. 248:1-14