UL-754

Material Data Sheet

SUMO2 Biotin

Price: $80.00
Catalog #: UL-754

SUMO2 Biotin

Price: 
$80
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-754 biotSUMO2_.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.

MW: 
10.6 kDa
Purity: 
> 95% by SDS-PAGE

SUMO-2 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. Biotinylated SUMO-2 can be detected using avidin-linked reagents for higher efficiency and sensitivity than with antibodies.

Use & Storage

Use: 
Depending on experimental conditions and detection method, suggested in
vitro concentrations for thiolester formation is 5 to 20 μM.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Desterro, J.M. et al. (1997) FEBs.Lett. 417:297-300
Okama T. et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat.Rev. 4:690-699
Su H-L.et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J.Biol.Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene 248:1-14