UL-753

Material Data Sheet

His6-SUMO2

Price: $150.00
Catalog #: UL-753

His6-SUMO2

Price: 
$150
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL753_hisSUMO2.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.

MW: 
11.3 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6 SUMO-2 (SMT3A)

The ubiquitin-like SUMO-2 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-2 is derived from the precursor pro-SUMO-2 (Accession # NM_006937). Human SUMO-2 shares 44% and 86% identity with SUMO-1 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Use & Storage

Use: 
Typical in vitro concentrations are 10-50 μM depending on conditions. 
Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380, 1993.
Dai K.-S. and Liew, C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Kamitani T. et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V., et al. (1997) Genomics 40: 362-366,
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Su H.-L. and Li S.S.-L. (2002) Gene 296: 65-73
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Yeh E.T.H., et al. (2000) Gene 248:1-14