UL-752

Material Data Sheet

SUMO2

Price: $159.00
Catalog #: UL-752

SUMO2

Price: 
$159
Quantity: 
500 µg
Data Sheet: 

Also known as: SUMO-2 (SMT3A)

The ubiquitin-like SUMO-2 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-2 is derived from the precursor pro-SUMO-2 (Accession # NM_006937). Human SUMO-2 shares 44% and 86% identity with SUMO-1 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
MW: 
11.6 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.

Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380, 1993.
Dai K.-S. and Liew, C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Kamitani T. et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V., et al. (1997) Genomics 40: 362-366,
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Su H.-L. and Li S.S.-L. (2002) Gene 296: 65-73
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Yeh E.T.H., et al. (2000) Gene 248:1-14