UL-752

Material Data Sheet

SUMO2

Price: $159.00
Catalog #: UL-752

SUMO2

Price: 
$159.00
Quantity: 
500 µg
Data Sheet: 

 

The ubiquitin-like SUMO2 is conjugated to a variety of proteins in the presence of UBE2I (Ubc9) and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO2 is derived from the precursor pro-SUMO2 (Accession # NM_006937). Human SUMO2 shares 44% and 86% identity with SUMO1 and SUMO3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol, 1mM DTT
MW: 
11 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.

Storage: 

Store at -80°C once reconstituted. Avoid multiple freeze/ thaw cycles.