UL-751

Material Data Sheet

His6-Pro-SUMO2

Price: $150.00
Catalog #: UL-751

His6-Pro-SUMO2

Price: 
$150
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL751_hisproSUMO2.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.

MW: 
13 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6 Pro-SUMO-2 (SMT3A)

All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-2 (95 amino acids) is the inactive precursor of SUMO-2 (93 amino acids) and is processed at the C-terminus by SUMO-2 specific proteases (SENPs). The resulting SUMO-2 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumolyation reactions or as a substrate for SENPs. NCBI accession # NM_006937.

Use & Storage

Use: 
Typical in vitro concentrations are 10-50 μM depending on conditions. 
Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380, 1993.
Dai K.-S. and Liew, C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Kamitani T. et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V., et al. (1997) Genomics 40: 362-366,
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Su H.-L. and Li S.S.-L. (2002) Gene 296: 65-73
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Yeh E.T.H., et al. (2000) Gene 248:1-14