UL-750

Material Data Sheet

SUMO2

Price: $175.00

Catalog #: UL-750

SUMO2

Price:

$175

Quantity:

500 μg

Data Sheet:

UL750_proSUMO2.pdf

Stock:

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.

MW:

10.9 kDa

Purity:

> 95% by SDS-PAGE

Also known as: Pro-SUMO-2 (SMT3A)

All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-2 (95 amino acids) is the inactive precursor of SUMO-2 (93amino acids) and is processed at the C-terminus by SUMO-2 specific proteases (SENPs). The resulting SUMO-2 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumoylation reactions or as a substrate for SENPs. NCBI Accession # NM_006937.

Use & Storage

Use:

Typical in vitro concentrations are 10-50 μM depending on conditions.

Storage:

SUMO1-agarose can be re-used for at least 10-15 applications if properly maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl pH9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN3 or 10-20 % ethanol. Resin should be stored at 4°C. DO NOT FREEZE

Literature

References:

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380, 1993.
Dai K.-S. and Liew, C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Kamitani T. et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V., et al. (1997) Genomics 40: 362-366,
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Su H.-L. and Li S.S.-L. (2002) Gene 296: 65-73
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Yeh E.T.H., et al. (2000) Gene 248:1-14