UL-740

Material Data Sheet

SUMO1 agarose

Price: $150.00
Catalog #: UL-740

SUMO1 agarose

Price: 
$150
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconUL-740_SUMOaga.pdf
Stock: 

0.5 ml SUMO1 agarose is supplied in a 1 ml total volume of 20% ethanol.

SUMO-1 is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of SUMO-1 activating enzyme, SUMO conjugating E2 enzyme Ubch9, SUMO-1 ligases, SUMO C-terminal hydrolases, and other proteins/enzymes that have an affinity for SUMO-1. The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of Ubch9 and the SAE1/SAE2 activating enzyme. SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 
SUMO1-agarose can be re-used for at least 10-15 applications if properly<br />maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl<br />pH9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM<br />HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN<sub>3</sub> or 10-20 %<br />ethanol. Resin should be stored at 4°C. DO NOT FREEZE

Literature

References: 

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem. Biophys. Res. Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat. Rev. 4:690-699
Su H-L. et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J. Biol. Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene 248:1-14