UL-736

Material Data Sheet

SUMO1 Rhodamine

Price: $175.00

Catalog #: UL-736

SUMO1 Rhodamine

Price:

$175

Quantity:

50 μg

Data Sheet:

UL-736 RhoSUMO1_.pdf

Stock:

X mg/ml (X μM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.

MW:

11.1 kDa

Purity:

> 95% by SDS-PAGE

SUMO-1 modified with rhodamine red via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. This labeled SUMO-1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies.

Use & Storage

Use:

Rhodamine SUMO-1 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex₅₇₀ nm and Em₅₉₀ nm wavelengths respectively.

Storage:

Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References:

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat.Rev. 4:690-699
Su H-L. et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J.Biol.Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene 248:1-14