UL-735

Material Data Sheet

SUMO1 Fluorescein

Price: $195.00
Catalog #: UL-735

SUMO1 Fluorescein

Price: 
$195
Quantity: 
50 µg

SUMO-1 modified with fluorescein via primary amine coupling. This results in multiple fluoresceinated SUMO-1 species with modified lysines as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to efficiently incorporate into poly-SUMO chains.

Product Information

Stock: 
X mg/ml (X μM) in 10 mM Hepes pH 8.0. Actual concenration will vary with specific Lot #.
MW: 
11.1 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein-SUMO gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat.Rev. 4:690-699
Su H-L. et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J.Biol.Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene 248:1-14