UL-725

Material Data Sheet

SUMO1 Biotin

Price: $80.00

Catalog #: UL-725

SUMO1 Biotin

Price:

$80

Quantity:

50 μg

Data Sheet:

UL725_biotSUMO.pdf

Stock:

X mg/ml (X μM) in 10mM Hepes pH8.0. Actual concentration will vary with specific Lot #.

MW:

11.1 kDa

Purity:

> 95% by SDS-PAGE

SUMO-1 modified with biotin via primary amine coupling. This results in multiple biotinylated SUMO-1 species modified at the N-terminus, as well as lysine residues. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly- SUMO chains. Biotinylated SUMO-1 can be detected using avidin-linked reagents.

Use & Storage

Use:

Suggested in vitro concentrations for thiolester formation with UbcH9
is 5 – 20 μM.

Storage:

Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References:

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300Okama T. et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698Seeler J-S. and Dejean A. (2003) Nat.Rev. 4:690-699Su H-L. et al. (2002) Gene 296:65-73Tatham M.H. et al. (2001) J.Biol.Chem. 276:35368-35374Yeh E.T.H. et al. (2000) Gene 248:1-14