UL-720

Material Data Sheet

Methylated SUMO1

Price: $195.00
Catalog #: UL-720

Methylated SUMO1

Price: 
$195
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL720_methSUMO.pdf
Stock: 

Soluble in aqueous buffers up to 5 mg/ml.

MW: 
11.1 kDa
Purity: 
> 95% by SDS-PAGE

The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-1 is derived from the precursor pro-SUMO-1 (Accession # NM_003352). Reductive methylation of lysine residues renders SUMO unable to form poly-SUMO chains via lysine linkages with other SUMO molecules. Ideal for the reduction in poly-SUMO chain length and rates of SUMO conjugation, or the demonstration of substrate mono- SUMOylation.

Use & Storage

Use: 
Typical in vitro concentrations are 10-50 μM depending on conditions. 
Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat.Rev. 4:690-699
Su H-L. et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J.Biol.Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene 248:1-14