Material Data Sheet
Also known as: SUMO-1 (SMT3C)
The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-1 is derived from the precursor pro-SUMO-1 (Accession # NM_003352). Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
Purity:> 95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Desterro, J.M. et al. (1997) FEBs. Lett. 417:297-300
Okama T. et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698
Seeler J-S. and Dejean A. (2003) Nat.Rev. 4:690-699
Su H-L. et al. (2002) Gene 296:65-73
Tatham M.H. et al. (2001) J.Biol.Chem. 276:35368-35374
Yeh E.T.H. et al. (2000) Gene 248:1-14