UL-705

Material Data Sheet

His6-Pro-SUMO1

Price: $150.00
Catalog #: UL-705

His6-Pro-SUMO1

Price: 
$150
Quantity: 
500 μg
Data Sheet: 
application/pdf iconUL705_hisproSUMO1.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.

MW: 
13.7 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6-Pro-SUMO-1 (SMT3C)

All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-1 (101 amino acids) is the inactive precursor of SUMO-1 (97 amino acids) and is processed at the C-terminus by SUMO-1 specific proteases (SENPs). The resulting SUMO-1 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumolyation reactions or as a substrate for SENPs. This His6 tag is at the N-terminus. NCBI accession # NM_003352.

Use & Storage

Use: 

Typical in vitro concentrations are 10-50 μM depending on conditions. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380, 1993.
Dai K.-S. and Liew C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Kamitani T. et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V., et al. (1997) Genomics 40: 362-366,
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Su H.-L. and Li S.S.-L. (2002) Gene 296: 65-73
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Yeh E.T.H., et al. (2000) Gene 248:1-14