Material Data Sheet
Substrate for SUMO deconjugating enzymes (SENPs) based on the C-terminal derivative of SUMO with aminoluciferin (AML). Rather than fluorescence as the indicator of SENP activity, SENP liberated luciferin is processed by luciferase to give a luminescence signal.
SUMO-AML not only produces a stronger signal, but also has an excellent signal to noise ratio over traditional fluorophores. This makes it possible to rapidly assess the activity of SENPs that poorly utilize SUMO-AMC while using much lower levels of the SENPs themselves.
Stock:X mg/ml (X mM) in 50mM HEPES pH 7.5, 100mM NaCl
>100:1 signal to noise ratio with 10 pM SENP1. Km of SENP1 for SUMO-1-AML is 88nM
Use & Storage
SUMO-AML gives a strong luminescent signal in the range of 0.1-1 µM, depending on experimental conditions. Optimal luminescence at pH 7.5 is monitored using all wavelengths with a 500 ms integration time.
Store at -80°C. Avoid multiple freeze/ thaw cycles.
Reddy G., et al. (2010) JACS. 132:39, 13586-13587
Shinde R., et al. (2006) Biochemistry 45 : 11103-11112