UL-704

Material Data Sheet

SUMO1 Aminoluciferin

Price: $215.00
Catalog #: UL-704

SUMO1 Aminoluciferin

Price: 
$215
Quantity: 
50 µg

 

Substrate for SUMO deconjugating enzymes (SENPs) based on the C-terminal derivative of SUMO with aminoluciferin (AML). Rather than fluorescence as the indicator of SENP activity, SENP liberated luciferin is processed by luciferase to give a luminescence signal. 

SUMO-AML not only produces a stronger signal, but also has an excellent signal to noise ratio over traditional fluorophores. This makes it possible to rapidly assess the activity of SENPs that poorly utilize SUMO-AMC while using much lower levels of the SENPs themselves.

Product Information

Stock: 
X mg/ml (X mM) in 50mM HEPES pH 7.5, 100mM NaCl
MW: 
11.3 kDa
Purity: 
> 95%
Activity: 

 

>100:1 signal to noise ratio with 10 pM SENP1. Km of SENP1 for SUMO-1-AML is 88nM

Use & Storage

Use: 

SUMO-AML gives a strong luminescent signal in the range of 0.1-1 µM, depending on experimental conditions.  Optimal luminescence at pH 7.5 is monitored using all wavelengths with a 500 ms integration time. 

Storage: 
Store at -80°C. Avoid multiple freeze/ thaw cycles.

Literature

References: 

 

Reddy G., et al. (2010) JACS. 132:39, 13586-13587

Shinde R., et al. (2006) Biochemistry 45 : 11103-11112