Substrate for SUMO deconjugating enzymes (SENPs) based on the C-terminal derivative of SUMO with aminoluciferin (AML). Rather than fluorescence as the indicator of SENP activity, SENP liberated luciferin is processed by luciferase to give a luminescence signal. 

SUMO-AML not only produces a stronger signal, but also has an excellent signal to noise ratio over traditional fluorophores. This makes it possible to rapidly assess the activity of SENPs that poorly utilize SUMO-AMC while using much lower levels of the SENPs themselves.