UL-703

Material Data Sheet

HA-SUMO1 Vinyl Sulfone

Price: $250.00
Catalog #: UL-703

HA-SUMO1 Vinyl Sulfone

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL703 HA-SUMO1 VS.pdf
Stock: 

X mg/ml (XμM) in 50 mM Hepes pH 7.0, 150 mMNaCl, 10 % glycerol. Concentration varies with Lot #.

MW: 
11.1 kDa
Purity: 
> 90%

Also known as: HA-SUMO1-VS

This N-terminal HA-tagged SUMO is a potent, irreversible and specific inhibitor of SUMO-specific proteases (SENPs). This protein inhibits the hydrolysis of poly-SUMO chains on substrate proteins in vitro and thus enhances poly-SUMO chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of SENP activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 μM. 

Storage: 
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291
Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95
Kessler B.M. (2006) Exp.Rev.Proteomics. 3 :213-221
Love K.R, et al. (2007) Nat.Chem.Biol. 3:697-705
Wang G., et al. (2003) Org.Lett. 5: 737-740