UL-620

Material Data Sheet

His6-Pro-ISG15

Price: $159.00
Catalog #: UL-620

His6-Pro-ISG15

Price: 
$159
Quantity: 
500 µg

Also known as: UCRP

The ubiquitin-like ISG15 is conjugated to intracellular target proteins. This pathway is distinct from that of ubiquitination with different substrate specificities and interactions with ligating enzymes. Pro-ISG15 (165 amino acids) is the inactive precursor of ISG15 (158 amino acids). The precursor is processed at the C-terminus by an ISG15-specific protease UBP43 (also known as USP18). The mature form of ISG15 contains the conserved C-terminal di-glycine residues which are critical in activation and conjugation reactions. This protein can be used as a negative control in ISGylation reactions or as a substrate for UBP43.
Note: His6 tag is on the C-terminus of the protein.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual protein concentration will vary with specific Lot #.
MW: 
18.8 kDa
Purity: 
> 95% by SDS-PAGE
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10:2346-2365
Kim K.I. and Zhang D. (2003) Biochem. Biophys. Res. Comm. 307:431-434
Knight E., et al. (1988) J. Biol Chem. 263:4520-4522
Loeb K.R and Haas A.L. (1992) J. Biol Chem. 267:7806-7813
Malakhov M.P. (2002) J. Biol Chem. 277:9976-9981
Potter J.L., et. al. (1999) J. Biol Chem. 267:25061-25068
Ritchie K.J and Zhang D.E. (2004) Sem. Cell. Dev. Bio. 2:237-246