Material Data Sheet
Also known as: UCRP
The ubiquitin-like ISG15 is conjugated to intracellular target proteins. This pathway is distinct from that of ubiquitination with different substrate specificities and interactions with ligating enzymes. Pro-ISG15 (165 amino acids) is the inactive precursor of ISG15 (158 amino acids). The precursor is processed at the C-terminus by an ISG15-specific protease UBP43 (also known as USP18). The mature form of ISG15 contains the conserved C-terminal di-glycine residues which are critical in activation and conjugation reactions. This protein can be used as a negative control in ISGylation reactions or as a substrate for UBP43.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual protein concentration will vary with specific Lot #.
Purity:> 95% by SDS-PAGE
Store at -80°C. Avoid multiple freeze/thaw cycles.
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