UL-613

Material Data Sheet

ISG15 Fluorescein

Price: $195.00
Catalog #: UL-613

ISG15 Fluorescein

Price: 
$195
Quantity: 
50 µg

ISG15 modified with fluorescein via primary amine coupling, resulting in the modification of lysine residues as well as the N-terminus. This labeled ISG15 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
17 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein-ISG15 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10: 2346-2365
Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10: 2701-2722
D’Cunha J., et al. (1996) Proc. Natl. Acad. Sci. 93: 211-215
Jentsch S. and Pyrowolakis G. (2000) Tren. Cell. Biol. 10: 335-342
Kim K.I. and Zhang D. (2003) Biochem. Biophys. Res. Comm. 307: 431-434
Liakopoulos D., et al. (1998) EMBO J. 17:2208-2214
Owhashi M., et al. (2003) Biochem. Biophys. Res. Comm. 309: 533-539
Narasimhan J., et al. (2005) J. Biol Chem. 280 : 27356-27365
Potter J.L., et al. (1999) J. Biol Chem. 267: 25061068
Ritchie K.J. and Zhang D.E. (2004) Sem. Cell. Dev. Biol. 2: 237-246