UL-612

Material Data Sheet

ISG15 Biotin

Price: $80.00
Catalog #: UL-612

ISG15 Biotin

Price: 
80
Quantity: 
50 µg
Data Sheet: 
application/pdf iconul-612-isg15-biotin.pdf

ISG15 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated ISG15 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
17 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Depending on experimental conditions and detection method, suggested in vitro concentrations for thiolester formation is 1 to 5 μM.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10: 2346-2365
Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10: 2701-2722
D’Cunha J., et al. (1996) Proc. Natl. Acad. Sci. 93: 211-215
Jentsch S. and Pyrowolakis G. (2000) Tren. Cell. Biol. 10: 335-342
Kim K.I. and Zhang D. (2003) Biochem. Biophys. Res. Comm. 307: 431-434
Liakopoulos D., et al. (1998) EMBO J. 17:2208-2214
Owhashi M., et al. (2003) Biochem. Biophys. Res. Comm. 309: 533-539
Narasimhan J., et al. (2005) J. Biol Chem. 280 : 27356-27365
Potter J.L., et al. (1999) J. Biol Chem. 267: 25061068
Ritchie K.J. and Zhang D.E. (2004) Sem. Cell. Dev. Biol. 2: 237-246