UL-610

Material Data Sheet

His6-ISG15

Catalog #: UL-610

His6-ISG15

Datasheet: 
application/pdf iconUL610_hisISG15.pdf
Product Source: 

human recombinant

Also known as: UCRP

The ubiquitin-like ISG15 is conjugated to intracellular target proteins. This pathway is distinct from that of ubiquitination with different in substrate specificity and interaction with ligating enzymes. ISG15 becomes conjugated to a diverse set of proteins after IFN-α/β stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. It may also serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments and may also be involved in autocrine, paracrine and endocrine mechanisms.

Product Information
Quantity: 

500 μg

Price: 

$150

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual protein concentration will vary with specific Lot #.

MW: 

17.9 kDa

Purity: 

> 95% by SDS-PAGE

Use & Storage
Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10: 2346-2365
Dao C.T. and Zhang D.E. (2005) Front. Biosci. 10: 2701-2722
D’Cunha J., et al. (1996) Proc. Natl. Acad. Sci. 93: 211-215
Jentsch S. and Pyrowolakis G. (2000) Tren. Cell. Biol. 10: 335-342
Kim K.I. and Zhang D. (2003) Biochem. Biophys. Res. Comm. 307: 431-434
Liakopoulos D., et al. (1998) EMBO J. 17:2208-2214
Owhashi M., et al. (2003) Biochem. Biophys. Res. Comm. 309: 533-539
Narasimhan J., et al. (2005) J. Biol Chem. 280 : 27356-27365
Potter J.L., et al. (1999) J. Biol Chem. 267: 25061068
Ritchie K.J. and Zhang D.E. (2004) Sem. Cell. Dev. Biol. 2: 237-246