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Material Data Sheet
HA-ISG15 Vinyl Sulfone
Price: $250.00
HA-ISG15 Vinyl Sulfone
Price:
250
Quantity:
50 µg
Data Sheet:
This N-terminal HA-tagged ISG15 is a potent, irreversible and specific inhibitor of ISG15-specific isopeptidases (such as UBP43, Cat # E-600). This protein blocks the hydrolysis of poly-ISG15 chains on substrate proteins in vitro and thus enhances poly-ISG15 chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of such deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.
Product Information
Stock:
X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol. Concentration varies with Lot #.
MW:
18.5 kDa
Purity:
> 90% Use & Storage
Use:
Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit ISG15 deconjugating enzymes in vitro are 1-5 μM.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.
Literature
References:
Borodovsky A, et al. (2002) Chem. Biol. 9:1149-1159
Galardy P., et al. (2005) Meth. Enz. 399:120-131
Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95
Linder H.A., et al. (2005) J. Virol. 79:15199-151208
Malakhov M.P, et al. (2002) J. Biol. Chem. 277:9976-9981
Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51
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