UL-602

Material Data Sheet

ISG15 Aldehyde

Price: $250.00
Catalog #: UL-602

ISG15 Aldehyde

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL602_ISG15aldehyde.pdf
Stock: 

X mg/ml (X7μM) in 50 mM Hepes pH 6.5, 200 mM NaCl, 10 % glycerol. Actual concentration will vary with specific Lot #. 

MW: 
17 kDa 
Purity: 
95% by HPLC 

Also known as: ISG15-H

A potent and highly specific inhibitor of ISG15-specific isopeptidases (such as UBP43). This protein blocks the hydrolysis of poly-ISG15 chains on substrate proteins in vitro and thus enhances poly-ISG15 chain accumulation.

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit ISG15 deconjugating enzymes in vitro are 1-5 μM. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles. 

Literature

References: 

Borodovsky A, et al. (2002) Chem. Biol. 9:1149-1159
Galardy P., et al. (2005) Meth. Enz. 399:120-131
Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95
Linder H.A., et al. (2005) J. Virol. 79:15199-151208
Malakhov M.P, et al. (2002) J. Biol. Chem. 277:9976-9981
Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51