UL-553

Material Data Sheet

ISG15 AMC

Price: $250.00
Catalog #: UL-553

ISG15 AMC

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL553_ISG15amc.pdf
Stock: 

X mg/ml (XμM) in 50 mM Hepes pH 6.5, 200 mM NaCl, 10 % glycerol. Actual concentration will vary with specific Lot #.

MW: 
17 kDa
Purity: 
> 95% by HPLC

Fluorogenic substrate for ISG15 hydrolases based on the carboxy-terminus derivatization of ISG15 with 7-amido-4-methylcoumarin (AMC). ISG15 AMC is useful for studying enzymes (such as UBP43) when detection sensitivity or continuous monitoring of activity is essential.

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM depending on conditions. Release of AMC fluorescence can be monitored using Ex380 nm and Em460 nm wavelengths respectively.

Purity: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A, et al. (2002) Chem. Biol. 9:1149-1159
Galardy P., et al. (2005) Meth. Enz. 399:120-131
Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95
Malakhov M.P, et al. (2002) J. Biol. Chem. 277:9976-9981
Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51