UL-552

Material Data Sheet

NEDD8 AMC

Price: $250.00
Catalog #: UL-552

NEDD8 AMC

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL552_Nedd8amc.pdf
Stock: 

X mg/ml (X μM) in 50 mM MOPS 6.5, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
9 kDa
Purity: 
> 95% by HPLC

Fluorogenic substrate for NEDD8 hydrolases based on the carboxy-terminus derivatization of NEDD8 with 7-amido-4-methylcoumarin (AMC). NEDD8 AMC is useful for studying NEDD8 hydrolases when detection sensitivity or continuous monitoring of activity is essential.

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM. Release of AMC fluorescence can be monitored using Ex380 nm and Em460 nm wavelengths respectively.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gan-Erdene T., et al. (2003) J. Biol. Chem. 278:28892-28900
Wu K, et al. (2003) J. Biol. Chem. 278:28882-288891