UL-551

Material Data Sheet

SUMO1 AMC

Price: $275.00
Catalog #: UL-551

SUMO1 AMC

Price: 
$275
Quantity: 
50 µg
Data Sheet: 

Fluorogenic substrate for SUMO-1 hydrolases based on the carboxy-terminus derivatization of SUMO-1 with 7-amido-4-methylcoumarin (AMC). SUMO-1 AMC is useful for studying SUMO-1 hydrolases when detection sensitivity or continuous monitoring of activity is essential.

Product Information

Stock: 
X mg/ml (XμM) in 100 % DMSO. Actual concentration will vary with specific Lot #.
MW: 
11.6 kDa
Purity: 
> 95% by HPLC

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM. Release of AMC
fluorescence can be monitored using Ex380 nm and Em460 nm wavelengths
respectively.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kim I.L., et al. (2000) J.Biol.Chem. 275:14102-14106
Suzuki T., et al. (1999) J.Biol.Chem. 274:31131-31134
Li S-J. and Hochstrasser M. (2003) J.Cell.Biol. 160:1069-1081