UL-551

Material Data Sheet

SUMO1 AMC

Price: $250.00

Catalog #: UL-551

SUMO1 AMC

Price:

$250

Quantity:

50 μg

Data Sheet:

UL551_SUMOamc.pdf

Stock:

X mg/ml (XμM) in 100 % DMSO. Actual concentration will vary with specific Lot #.

MW:

11.6 kDa

Purity:

> 95% by HPLC

Fluorogenic substrate for SUMO-1 hydrolases based on the carboxy-terminus derivatization of SUMO-1 with 7-amido-4-methylcoumarin (AMC). SUMO-1 AMC is useful for studying SUMO-1 hydrolases when detection sensitivity or continuous monitoring of activity is essential.

Use & Storage

Use:

Substrate concentrations for assay range from 0.1-1 μM. Release of AMC
fluorescence can be monitored using Ex₃₈₀ nm and Em₄₆₀ nm wavelengths
respectively.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References:

Kim I.L., et al. (2000) J.Biol.Chem. 275:14102-14106
Suzuki T., et al. (1999) J.Biol.Chem. 274:31131-31134
Li S-J. and Hochstrasser M. (2003) J.Cell.Biol. 160:1069-1081