UL-530

Material Data Sheet

UFM1 agarose

Price: $150.00
Catalog #: UL-530

UFM1 agarose

Price: 
$150
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconUL530_Ufm1 agarose.pdf
Stock: 

 0.5 ml Ufm1 agarose is supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

UFM1 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of UFM1 interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and
elution of material is dependent on individual experimental conditions.

Storage: 
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1M KCL. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be used as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Kang, S.H. et al. (2006) J. Biol. Chem. 282: 5256-5262
Komatsu,M., et al. (2004) EMBO J. 23: 1977-1986
Sasakawa, H., et al. (2006) Biochem. Biophys. Res. Commun. 343: 21-26