UL-522

Material Data Sheet

Ufm1 Rhodamine

Price: $175.00
Catalog #: UL-522

Ufm1 Rhodamine

Price: 
$175
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-522 RhoUfm1.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.

MW: 
12.1 kDa
Purity: 
> 95% by SDS-PAGE

UFM1 modified withrhodamine red via primary amine coupling, resulting in the modification of lysine residues as well as the N-terminus. This labeled UFM1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.

Use & Storage

Use: 
Rhodamine-UFM1 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored
using Ex570 nm and Em590 nm wavelengths respectively. 
Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kang, S.H. et al. (2006) J. Biol. Chem. 282: 5256-5262
Komatsu,M., et al. (2004) EMBO J. 23: 1977-1986
Sasakawa, H., et al. (2006) Biochem. Biophys. Res. Commun. 343: 21-26