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Material Data Sheet
UFM1 Biotin
Price: $80.00
UFM1 Biotin
Price:
$80
Quantity:
50 μg
Data Sheet:
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.
MW:
12.1 kDa
Purity:
> 95% by SDS-PAGE UFM1 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated Ufm1 can be detected using avidinlinked reagents for higher efficiency and detection sensitivity than with antibodies.
Use & Storage
Use:
Depending on experimental conditions and detection method, suggested in vitro concentrations for thiolester formation is 1 to 5 μM.
Storage:
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles. Literature
References:
Kang, S.H. et al. (2006) J. Biol. Chem. 282: 5256-5262
Komatsu,M., et al. (2004) EMBO J. 23: 1977-1986
Sasakawa, H., et al. (2006) Biochem. Biophys. Res. Commun. 343: 21-26






