UL-520

Material Data Sheet

UFM1 Biotin

Price: $175.00
Catalog #: UL-520

UFM1 Biotin

Price: 
175
Quantity: 
50 µg
Data Sheet: 
application/pdf iconul-520-ufm1-biotin.pdf
Data sheet linked from R&D Systems: 
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UFM1 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated Ufm1 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.
MW: 
12.1 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Depending on experimental conditions and detection method, suggested in vitro concentrations for thiolester formation is 1 to 5 μM.

Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kang, S.H. et al. (2006) J. Biol. Chem. 282: 5256-5262
Komatsu,M., et al. (2004) EMBO J. 23: 1977-1986
Sasakawa, H., et al. (2006) Biochem. Biophys. Res. Commun. 343: 21-26