UL-520

Material Data Sheet

UFM1 Biotin

Price: $80.00
Catalog #: UL-520

UFM1 Biotin

Price: 
$80
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-520 biot Ufm1_.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.

MW: 
12.1 kDa
Purity: 
> 95% by SDS-PAGE
UFM1 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated Ufm1 can be detected using avidinlinked reagents for higher efficiency and detection sensitivity than with antibodies.

Use & Storage

Use: 
Depending on experimental conditions and detection method, suggested in vitro concentrations for thiolester formation is 1 to 5 μM.
Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kang, S.H. et al. (2006) J. Biol. Chem. 282: 5256-5262
Komatsu,M., et al. (2004) EMBO J. 23: 1977-1986
Sasakawa, H., et al. (2006) Biochem. Biophys. Res. Commun. 343: 21-26