UL-505

Material Data Sheet

His6-Pro UFM1

Price: $159.00
Catalog #: UL-505

His6-Pro UFM1

Price: 
$159
Quantity: 
250 µg
Data Sheet: 

UFM1 (Ubiquitin-Fold Modifier 1) is a ubiquitin-like protein that is covalently linked to target proteins via enzymatic reactions similar to ubiquitin. UFM1 is derived from the precursor form pro-UFM1 (Accession # NP_057701) which contains two additional amino acids (Ser84Cys85) following the conserved Gly83. While the sequence of UFM1 shares only 16% sequence identify with ubiquitin, they have similar overall tertiary structures. Mature UFM1 is activated by the E1-like enzyme Uba5 (E-319) and subsequently transferred to its cognate E2-like conjugating enzyme, Ufc1 (E2-675). It is known that UFM1 becomes conjugated to as yet unidentified proteins in HEK cells and various mouse tissues. UFM1 is conserved in metazoa and plants, but not yeast. The exact cellular function and role of UFM1 modification in vivo is not yet known.
This protein contains an N-terminal His6 tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.
MW: 
12.2 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions. 

Storage: 
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kang, S.H. et al. (2006) J. Biol. Chem. 282: 5256-5262
Komatsu,M., et al. (2004) EMBO J. 23: 1977-1986
Liu G., et al. (2009) J. Struct. Funct. Genomics. 10:127-136
Sasakawa, H., et al. (2006) Biochem. Biophys. Res. Commun. 343: 21-26