UL-455

New
Material Data Sheet

MAP1LC3A-Rhodamine110

Price: $399.00
Catalog #: UL-455

MAP1LC3A-Rhodamine110

Price: 
$399
Quantity: 
50 µg
Data Sheet: 

MAP1LC3A-Rhodamine110, human recombinant

Microtubule-associated proteins 1A/1B light chain 3A (also known as MAP1LC3A) is an Ubiquitin-like modifier of the ATG8 family and is involved in the formation of the autophagosomes.  This protein interacts directly with Sequestosome-1 and is recruited to inclusion bodies containing polyubiquitinated protein aggregates where it plays a role in inclusion body degradation by autophagy.  MAP1LC3A-Rhodamine110 (Rh110) is a fluorogenic substrate for the autophagy hydrolase ATG4B.  This substrate is useful for studying ATG4B when detection sensitivity or continuous monitoring of activity is essential.

Product Information

Stock: 
0.62 mg/ml (44 µM) in 50 mM MES, pH 6.0, 100 mM NaCl, 20% glycerol
MW: 
14 kDa
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 

MAP1LC3A-Rhodamine gives a strong signal in the range of 0.1-1 µM, depending on exact experimental conditions.  Optimal fluorescence at pH 8.0 is monitored using Ex485 nm and Em535 nm wavelengths respectively

For Laboratory Research Use Only, Not For Use in Humans

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.

Purity: 
> 90% by SDS-PAGE

Literature

References: 

Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159

Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850

Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221

Kumanomidou T., et al. (2006) J. Mol. Biol. 355 :612-618

Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705

Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51