UL-450

Material Data Sheet

HA-LC3/MAP1LC3A/Apg8p3 AMC

Price: $250.00
Catalog #: UL-450

HA-LC3/MAP1LC3A/Apg8p3 AMC

Price: 
$250
Quantity: 
25 μg
Data Sheet: 
application/pdf iconUL450 Apg8p3amc.pdf
Stock: 

 X mg/ml (X μM) in 50 mM Hepes pH 6.5, 100 mM NaCl, 10 % glycerol.Concentration varies with Lot #. 

MW: 
14.4 kDa
Purity: 
> 90%

 

Fluorogenic substrate for Apg8-specific C-terminal hydrolases such as Apg4b (Cat# E-400) based on the C-terminal derivatization of Apg8 with 7-amido-4-methylcoumarin (AMC). Apg8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.

Use & Storage

Use: 

 Substrate concentrations for assay range from 0.1-1 μM depending onconditions. Release of AMC fluorescence can be monitored using Ex380 nmand Em460 nm wavelengths respectively. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159

Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850

Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221

Kumanomidou T., et al. (2006) J. Mol. Biol. 355 :612-618

Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705

Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51