UL-450

Material Data Sheet

HA-LC3/MAP1LC3A/Apg8p3 AMC

Price: $250.00

Catalog #: UL-450

HA-LC3/MAP1LC3A/Apg8p3 AMC

Price:

250

Quantity:

25 µg

Data Sheet:

ul-450-ha-lc3map1lc3aapg8p3-amc.pdf

Fluorogenic substrate for Apg8-specific C-terminal hydrolases such as Apg4b (Cat# E-400) based on the C-terminal derivatization of Apg8 with 7-amido-4-methylcoumarin (AMC). Apg8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.

Product Information

Stock:

X mg/ml (X μM) in 50 mM Hepes pH 6.5, 100 mM NaCl, 10 % glycerol.Concentration varies with Lot #.

MW:

14.4 kDa

Purity:

> 90%

Use & Storage

Use:

Substrate concentrations for assay range from 0.1-1 μM depending onconditions. Release of AMC fluorescence can be monitored using Ex380 nmand Em460 nm wavelengths respectively.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.