Material Data Sheet
Fluorogenic substrate for Apg8-specific C-terminal hydrolases such as Apg4b (Cat# E-400) based on the C-terminal derivatization of Apg8 with 7-amido-4-methylcoumarin (AMC). Apg8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.
Stock:X mg/ml (X μM) in 50 mM Hepes pH 6.5, 100 mM NaCl, 10 % glycerol.Concentration varies with Lot #.
Use & Storage
Substrate concentrations for assay range from 0.1-1 μM depending onconditions. Release of AMC fluorescence can be monitored using Ex380 nmand Em460 nm wavelengths respectively.
Store at -80°C. Avoid multiple freeze/thaw cycles.
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