UL-446

Material Data Sheet

HA-GATE16 Vinyl Sulfone

Price: $269.00
Catalog #: UL-446

HA-GATE16 Vinyl Sulfone

Price: 
$269
Quantity: 
25 µg

This N-terminal HA-tagged Apg8 protein is a potent, irreversible and specific inhibitor of Agp8-specific isopeptidases (such as Apg84B, Cat E-400). Apg84B activities include the processing of Apg8 precursor proteins and the removal of Apg8 proteins that are conjugated to phosphatidylethanolamine during autophagy. These processes can be inhibited by this vinyl sulfone derivative which reacts with the Apg84B active site cysteine. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of such deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol. Concentration varies with Lot #.
MW: 
13.8 kDa
Purity: 
> 90%

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions and detection method, typical concentrations to fully inhibit Apg deconjugating enzymes in vitro are 1-5 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Borodovsky A, et al. (2002) Chem. Biol. 9:1149-1159

Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221

Kumanomidou T., et al. (2006) J. Mol. Biol. 355 :612-618

Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705

Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51