UL-441

Material Data Sheet

HA-GABARAP Vinyl Sulfone

Price: $269.00
Catalog #: UL-441

HA-GABARAP Vinyl Sulfone

Price: 
$269
Quantity: 
25 µg
Data Sheet: 

This N-terminal HA-tagged protein is a potent, irreversible and specific inhibitor of ATG-specific isopeptidases (such as ATG4B, Cat # E-400).  ATG4B activities include the processing of ATG8 precursor proteins and the removal of ATG8 proteins that are conjugated to phosphatidylethanolamine during autophagy.  These processes can be inhibited by this vinyl sulfone derivative which reacts with the ATG4B active site cysteine.  The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein.  This epitope allows for the sensitive identification or purification of such deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol. Concentration varies with Lot #.
MW: 
14 kDa
Purity: 
> 90%

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution.  Depending on conditions and detection method, typical concentrations to fully inhibit ATG8 deconjugating enzymes in vitro are 1-5 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Borodovsky A, et al. (2002) Chem. Biol. 9:1149-1159

Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850

Hemelaar J., et al. (2004) Mol. Cell. Biol. 24:84-95

Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221

Kumanomidou T., et al. (2006) J. Mol. Biol. 355 :612-618

Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705

Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51