UL-440
HA-GABARAP
HA-GABARAP
Fluorogenic substrate for Apg8-specific C-terminal hydrolases such as Apg4b (Cat# E-400) based on the C-terminal derivatization of Apg8 with 7-amido-4-methylcoumarin (AMC). Apg8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.
Product Information
Use & Storage
Substrate concentrations for assay range from 0.1-1 μM depending on conditions. Release of AMC fluorescence can be monitored using Ex380 nm and Em460 nm wavelengths respectively.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Literature
Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159
Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850
Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221
Kumanomidou T., et al. (2006) J. Mol. Biol.. 355 :612-618
Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705
Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51