UL-440

Material Data Sheet

HA-GABARAP

Price: $259.00
Catalog #: UL-440

HA-GABARAP

Price: 
$259
Quantity: 
25 µg
Data Sheet: 
application/pdf iconul-440-ha-gabarapapg8p1-amc.pdf

Fluorogenic substrate for Apg8-specific C-terminal hydrolases such as Apg4b (Cat# E-400) based on the C-terminal derivatization of Apg8 with 7-amido-4-methylcoumarin (AMC). Apg8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM Hepes pH 6.5, 100 mM NaCl, 10 % glycerol.
MW: 
14 kDa
Purity: 
> 90%

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM depending on conditions. Release of AMC fluorescence can be monitored using Ex380 nm and Em460 nm wavelengths respectively.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159

Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850

Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221

Kumanomidou T., et al. (2006) J. Mol. Biol.. 355 :612-618

Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705

Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51