Material Data Sheet

LC3/MAP1LC3A Agarose

Price: $159.00
Catalog #: UL-435

LC3/MAP1LC3A Agarose

500 µL
Data Sheet: 

MAPLC3A covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of MAP-LC3a interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the ATG8 E1 activating enzyme ATG7, the ATG8 conjugating enzyme ATG3, ATG8 processing enzyme ATG4 and other autophagy pathway proteins and enzymes.

Product Information

0.5 ml MAPLC3A agarose is supplied in a 1 ml total volume of 50 mM HEPES pH 7.5, 250 mM NaCl.

Use & Storage


Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions. 


The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.