UL-435

Material Data Sheet

LC3/MAP1LC3A Agarose

Price: $159.00
Catalog #: UL-435

LC3/MAP1LC3A Agarose

Price: 
$159
Quantity: 
500 µL
Data Sheet: 

MAPLC3A covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of MAP-LC3a interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the ATG8 E1 activating enzyme ATG7, the ATG8 conjugating enzyme ATG3, ATG8 processing enzyme ATG4 and other autophagy pathway proteins and enzymes.

Product Information

Stock: 
0.5 ml MAPLC3A agarose is supplied in a 1 ml total volume of 50 mM HEPES pH 7.5, 250 mM NaCl.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions. 

Storage: 

The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.