UL-435

Material Data Sheet

LC3/MAP1LC3A/Apg8p3 Agarose

Price: $150.00
Catalog #: UL-435

LC3/MAP1LC3A/Apg8p3 Agarose

Price: 
$150
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconUL-435_MAPLC3 agarose.pdf
Stock: 

0.5 ml Apg8p3 agaroseis supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

MAP-LC3a covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of MAP-LC3a interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the Apg8 E1 activating enzyme Apg7, the Apg8 conjugating enzyme Apg3, Apg8 processing enzyme Apg4 and other autophagy pathway proteins and enzymes.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions. 

Storage: 
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Hammerback J.A., et al. (1991) Neuron 7: 521-524
He H., et al. (2003) J.Biol.Chem. 278: 29278-29287
Langkopf A., et al. (1992) J.Biol.Chem. 267: 16561-16566
Kabeya Y., et al. (2000) EMBO J. 19: 5720-5728
Kabeya Y., et al. (2004) J. Cell. Sci. 117: 2805-2812
Mann S.S., et al. (1996) J. Neurosci. 43: 535-544
Mann S.S. and Hammerback J.A.. (1994) J. Biol. Chem. 269: 11492-11497
Sugawara K., et al. (2004) Genes. Cells. 9: 611-618