UL-434

Material Data Sheet

His6-LC3/MAP1LC3A/Apg8p3 Fluorescein 

Price: $175.00
Catalog #: UL-434

His6-LC3/MAP1LC3A/Apg8p3 Fluorescein 

Price: 
175
Quantity: 
50 µg
Data Sheet: 
application/pdf iconul-434-his6-lc3map1lc3aapg8p3-fluorescein .pdf

Apg8p3 modified with fluorescein via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated Apg8p3 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies. This protein is Nterminally His6-tagged.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
16.3 kDa
Purity: 
> 95% by PAGE

Use & Storage

Use: 

Fluorescein- His6-Apg8p3 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hammerback J.A., et al. (1991) Neuron 7: 521-524
He H., et al. (2003) J.Biol.Chem. 278: 29278-29287
Langkopf A., et al. (1992) J.Biol.Chem. 267: 16561-16566
Kabeya Y., et al. (2000) EMBO J. 19: 5720-5728
Kabeya Y., et al. (2004) J. Cell. Sci. 117: 2805-2812
Mann S.S., et al. (1996) J. Neurosci. 43: 535-544
Mann S.S. and Hammerback J.A.. (1994) J. Biol. Chem. 269: 11492-11497
Sugawara K., et al. (2004) Genes. Cells. 9: 611-618