UL-430

Material Data Sheet

His6-LC3/MAP1LC3A

Price: $159.00
Catalog #: UL-430

His6-LC3/MAP1LC3A

Price: 
$159
Quantity: 
500 µg

There are at least three groups of mammalian Apg8 proteins which are homologs of the yeast Atg8 protein, including MAP-LC3a (Microtubule-Associated Protein Light Chain-3) which is part of the LC3 subfamily. The mammalian Apg8 proteins are ubiquitin-like modifiers that have divergent functions in human, and are essential in autophagic conjugation systems. This modifier protein has a conserved C-terminal glycine residue that becomes covalently attached to phosphatidylethanolamine (PE) after it is activated by the Apg7p (E1) and Apg3p (E2) enzymes. MAP-LC3 is the most abundant Apg8 homolog in autophagosomal membranes, and is thought to play a major role in mammalian autophagy. Accession # NP_115903.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.
MW: 
16.3 kDa
Purity: 
> 95% by PAGE

Use & Storage

Use: 

Typical concentration to support conjugation in vitro is 10-50 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hammerback J.A., et al. (1991) Neuron 7: 521-524
He H., et al. (2003) J.Biol.Chem. 278: 29278-29287
Langkopf A., et al. (1992) J.Biol.Chem. 267: 16561-16566
Kabeya Y., et al. (2000) EMBO J. 19: 5720-5728
Kabeya Y., et al. (2004) J. Cell. Sci. 117: 2805-2812
Mann S.S., et al. (1996) J. Neurosci. 43: 535-544
Mann S.S. and Hammerback J.A.. (1994) J. Biol. Chem. 269: 11492-11497
Sugawara K., et al. (2004) Genes. Cells. 9: 611-618