UL-426

Material Data Sheet

His6-GATE-16/Apg8p2 Rhodamine

Price: $175.00
Catalog #: UL-426

His6-GATE-16/Apg8p2 Rhodamine

Price: 
$175
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-426 RhoGATE16_.pdf
Stock: 

X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.

MW: 
15.7 kDa
Purity: 
> 95% by PAGE

His6-Apg8p2 modified with rhodamine red via primary amine coupling. This results in rhodamine coupled Apg8p2 species with modified lysines as well as the N-terminus. Detection of rhodamine-Apg8p2 occurs with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.

Use & Storage

Use: 
Rhodamine Apg8p2 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex570 nm and Em590 nm wavelengths respectively. 
Storage: 
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin by washing with 5 ml 50 mM Hepes pH 8.0, 1 M NaCl. Remove cleaning solution by washing resin with 10 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Becher P., et al. (2002) J. Virol. 76: 13069-13076
Elazar Z., et al. (2001) Genomics 74: 408-413
Paz Y., et al. (2000) J. Biol. Chem. 275: 25445-2545-
Sagiv Y., et al. (2000) EMBO J. 19: 1494-1504
Scherz-Shouval R., et al. (2003) J. Biol. Chem. 278: 14053-14058
Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744
Xin Y., et al. (2001) Genomics 74: 408-413