Material Data Sheet

GATE16 Agarose

Price: $0.00
Catalog #: UL-425

GATE16 Agarose

500 µL
Data Sheet: 

GATE 16 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of GATE 16 interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the Apg8 E1 activating enzyme Apg7, the Apg8 conjugating enzyme Apg3, Apg8 processing enzyme Apg4 and other autophagy pathway proteins and enzymes.

Product Information

0.5 ml GATE-16/Apg8p2 agarose is supplied in 1 ml total volume of 50 mM Hepes pH 8.0, 250 mM NaCl.

Use & Storage


Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.


The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin by washing with 5 ml 50 mM Hepes pH 8.0, 1 M NaCl. Remove cleaning solution by washing resin with 10 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.



Becher P. et al. (2002) J. Virol. 76: 13069-13076
Elazar Z. et al. (2001) Genomics 74: 408-413
Klionsky D.J. (2005) J. Cell. Sci. 118: 7-18
Paz Y. et al. (2000) J. Biol. Chem. 275: 25445-2545
Sagiv Y. et al. (2000) EMBO J. 19: 1494-1504
Scherz-Shouval R. et al. (2003) J. Biol. Chem. 278: 14053-14058
Tanida I. et al. (2002) J. Biol. Chem. 277: 13739-13744
Xin Y. et al. (2001) Genomics 74: 408-413