UL-425

Material Data Sheet

GATE-16/Apg8p2 Agarose

Price: $150.00
Catalog #: UL-425

GATE-16/Apg8p2 Agarose

Price: 
$150
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconUL-425.pdf
Stock: 

 0.5 ml GATE-16/Apg8p2 agarose is supplied in 1 ml total volume of 50 mM Hepes pH 8.0, 250 mM NaCl.

GATE-16 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of GATE-16 interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the Apg8 E1 activating enzyme Apg7, the Apg8 conjugating enzyme Apg3, Apg8 processing enzyme Apg4 and other autophagy pathway proteins and enzymes.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin by washing with 5 ml 50 mM Hepes pH 8.0, 1 M NaCl. Remove cleaning solution by washing resin with 10 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Becher P., et al. (2002) J. Virol. 76: 13069-13076
Elazar Z., et al. (2001) Genomics 74: 408-413
Paz Y., et al. (2000) J. Biol. Chem. 275: 25445-2545-
Sagiv Y., et al. (2000) EMBO J. 19: 1494-1504
Scherz-Shouval R., et al. (2003) J. Biol. Chem. 278: 14053-14058
Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744
Xin Y., et al. (2001) Genomics 74: 408-413